Journal: Journal of Extracellular Vesicles
Article Title: Outer Membrane Vesicles Mediate the Secretion and Nuclear Trafficking of a Bacterial Nucleomodulin
doi: 10.1002/jev2.70286
Figure Lengend Snippet: Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume BHI was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 µg/ml Pam3CSK4 was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Article Snippet: As controls, cells we co‐cultured with either BHI broth alone (negative) or Pam3CSK4 (1 μg/ml, w/v; InvivoGen; positive).
Techniques: Incubation, Mutagenesis, Bacteria, Positive Control, Produced, Negative Control
